› 5tfg

X-ray diffraction
1.91Å resolution

Nucleotide-binding domain 1 of the human cystic fibrosis transmembrane conductance regulator (CFTR) with 5-methyl-UTP

Released:
DOI: 10.2210/pdb5tfg/pdb
PDB entry 5tfg coloured by chain, front view.
PDB entry 5tfg coloured by chain, side view.
PDB entry 5tfg coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Wang C, Aleksandrov AA, Yang Z, Forouhar F, Proctor E, Kota P, An J, Kaplan A, Khazanov N, Boel G, Stockwell BR, Senderowitz H, Dokholyan NV, Riordan JR, Brouillette CG, Hunt JF

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146638 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystic fibrosis transmembrane conductance regulator Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 25.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P13569 (Residues: 387-646; Coverage: 15%)
Gene names: ABCC7, CFTR
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand M5T 1 x M5T
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P43
Unit cell:
a: 40.147Å b: 40.147Å c: 141.787Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.156 0.184
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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