› 5th6

X-ray diffraction
1.7Å resolution

Structure determination of a potent, selective antibody inhibitor of human MMP9 (apo MMP9)

Released:
DOI: 10.2210/pdb5th6/pdb
PDB entry 5th6 coloured by chain, front view.
PDB entry 5th6 coloured by chain, side view.
PDB entry 5th6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biochemical characterization and structure determination of a potent, selective antibody inhibitor of human MMP9.
Appleby TC, Greenstein AE, Hung M, Liclican A, Velasquez M, Villaseñor AG, Wang R, Wong MH, Liu X, Papalia GA, Schultz BE, Sakowicz R, Smith V, Kwon HJ
J Biol Chem 292 6810-6820 (2017)
PMID: 28235803

Function and Biology Details

Reaction catalysed: 
Cleavage of gelatin types I and V and collagen types IV and V.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147085 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
67 kDa matrix metalloproteinase-9 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 231 amino acids
Theoretical weight: 25.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14780 (Residues: 40-443; Coverage: 33%)
Gene names: CLG4B, MMP9
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand CA 12 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 76.546Å b: 69.233Å c: 84.093Å
α: 90° β: 101.77° γ: 90°
R-values:
R R work R free
0.208 0.208 0.235
Expression system: Escherichia coli

Quick links

Citations

17 review citations

The Rebirth of Matrix Metalloproteinase Inhibitors: Moving Beyond the Dogma.
Fields GB. (2019)
16 more

14 mentions without citation

Biochemical characterization and structure determination of a potent, selective antibody inhibitor of human MMP9.
Appleby et al. (2017)
13 more