› 5tj7

X-ray diffraction
2.6Å resolution

Structure of WWP2 WW2-2,3-linker-HECT aa 334-398 linked to 485-865

Released:
DOI: 10.2210/pdb5tj7/pdb
PDB entry 5tj7 coloured by chain, front view.
PDB entry 5tj7 coloured by chain, side view.
PDB entry 5tj7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A Tunable Brake for HECT Ubiquitin Ligases.
Chen Z, Jiang H, Xu W, Li X, Dempsey DR, Zhang X, Devreotes P, Wolberger C, Amzel LM, Gabelli SB, Cole PA
Mol Cell 66 345-357.e6 (2017)
PMID: 28475870

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126241 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NEDD4-like E3 ubiquitin-protein ligase WWP2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 447 amino acids
Theoretical weight: 53.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00308 (Residues: 334-865; Coverage: 51%)
Gene name: WWP2
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand NA 3 x NA
Ligand SCN 1 x SCN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ DW
Spacegroup: P1
Unit cell:
a: 72Å b: 73.907Å c: 82.38Å
α: 89.99° β: 89.66° γ: 90.01°
R-values:
R R work R free
0.211 0.207 0.284
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Regulating the human HECT E3 ligases.
Sluimer et al. (2018)
19 more

4 mentions without citation

A Tunable Brake for HECT Ubiquitin Ligases.
Chen et al. (2017)
3 more