› 5tl5

X-ray diffraction
1.8Å resolution

COMPLEX BETWEEN HUMAN CD27 AND ANTIBODY M2177

Released:
DOI: 10.2210/pdb5tl5/pdb
PDB entry 5tl5 coloured by chain, front view.
PDB entry 5tl5 coloured by chain, side view.
PDB entry 5tl5 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of CD27 in complex with a neutralizing noncompeting antibody.
Teplyakov A, Obmolova G, Malia TJ, Gilliland GL
Acta Crystallogr F Struct Biol Commun 73 294-299 (2017)
PMID: 28471362

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230910 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
M2177 LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 23.71 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
M2177 HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 229 amino acids
Theoretical weight: 24.44 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
CD27 antigen Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P26842 (Residues: 21-121; Coverage: 42%)
Gene names: CD27, TNFRSF7
Sequence domains: TNFR/NGFR cysteine-rich region

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand NAG 1 x NAG
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 274.2Å b: 37.41Å c: 61.08Å
α: 90° β: 98.82° γ: 90°
R-values:
R R work R free
0.197 0.196 0.232
Expression systems:
  • Homo sapiens
  • unidentified baculovirus

Quick links

Citations

4 citation in other articles

Extended clinical and immunological phenotype and transplant outcome in CD27 and CD70 deficiency.
Ghosh et al. (2020)
3 more

4 mentions without citation

Anti-Chikungunya Virus Monoclonal Antibody That Inhibits Viral Fusion and Release.
Tumkosit et al. (2020)
3 more