› 5toa

X-ray diffraction
2.5Å resolution

Crystal Structure of ER beta bound to Estradiol

Released:
DOI: 10.2210/pdb5toa/pdb
PDB entry 5toa coloured by chain, front view.
PDB entry 5toa coloured by chain, side view.
PDB entry 5toa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An alternative conformation of ERβ bound to estradiol reveals H12 in a stable antagonist position.
Souza PCT, Textor LC, Melo DC, Nascimento AS, Skaf MS, Polikarpov I
Sci Rep 7 3509 (2017)
PMID: 28615710

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187733 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Estrogen receptor beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 28.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92731 (Residues: 262-509; Coverage: 47%)
Gene names: ESR2, ESTRB, NR3A2
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

1 bound ligand:
Ligand EST 2 x EST
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P3221
Unit cell:
a: 83.811Å b: 83.811Å c: 168.531Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.244
Expression system: Escherichia coli

Quick links

Citations

1 review citation

A Novel Action of Endocrine-Disrupting Chemicals on Wildlife; DDT and Its Derivatives Have Remained in the Environment.
Matsushima A. (2018)
6 other articles

19 mentions without citation

Inhibiting the NLRP3 Inflammasome.
El-Sharkawy et al. (2020)
18 more