› 5tog

X-ray diffraction
1.08Å resolution

Room temperature structure of ubiquitin variant u7ub25.2540

Released:
DOI: 10.2210/pdb5tog/pdb
PDB entry 5tog coloured by chain, front view.
PDB entry 5tog coloured by chain, side view.
PDB entry 5tog coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Flexibility and Design: Conformational Heterogeneity along the Evolutionary Trajectory of a Redesigned Ubiquitin.
Biel JT, Thompson MC, Cunningham CN, Corn JE, Fraser JS
Structure 25 739-749.e3 (2017)
PMID: 28416112

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-143231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 78 amino acids
Theoretical weight: 8.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 151-228; Coverage: 34%)
Gene name: UBB
Sequence domains: Ubiquitin family

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3121
Unit cell:
a: 43.92Å b: 43.92Å c: 55.44Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.104 0.103 0.121
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Chemistry of Peptidoglycan in Mycobacterium tuberculosis Life Cycle: An off-the-wall Balance of Synthesis and Degradation.
Squeglia et al. (2018)
1 more

2 mentions without citation

Mapping the interaction surface of scorpion β-toxins with an insect sodium channel.
Zhorov et al. (2021)
1 more