5trz

X-ray diffraction
2.25Å resolution

Crystal structure of MHC-I H2-KD complexed with peptides of Mycobacterial tuberculosis (YQSGLSIVM)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-135303 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, K-D alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 275 amino acids
Theoretical weight: 32.16 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01902 (Residues: 23-297; Coverage: 79%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, D
Molecule details ›
Chains: B, D
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Diacylglycerol acyltransferase/mycolyltransferase Ag85B Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 9 amino acids
Theoretical weight: 997 Da
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided
UniProt:
  • Canonical: P9WQP1 (Residues: 102-110; Coverage: 3%)
Gene names: MTCY180.32, Rv1886c, fbpB

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 46.635Å b: 88.816Å c: 110.754Å
α: 90° β: 89.99° γ: 90°
R-values:
R R work R free
0.174 0.171 0.198
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided