› 5tud

X-ray diffraction
3Å resolution

Structural Insights into the Extracellular Recognition of the Human Serotonin 2B Receptor by an Antibody

Released:
DOI: 10.2210/pdb5tud/pdb
PDB entry 5tud coloured by chain, front view.
PDB entry 5tud coloured by chain, side view.
PDB entry 5tud coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the extracellular recognition of the human serotonin 2B receptor by an antibody.
Ishchenko A, Wacker D, Kapoor M, Zhang A, Han GW, Basu S, Patel N, Messerschmidt M, Weierstall U, Liu W, Katritch V, Roth BL, Stevens RC, Cherezov V
Proc Natl Acad Sci U S A 114 8223-8228 (2017)
PMID: 28716900

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230828 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
5-hydroxytryptamine receptor 2B; Soluble cytochrome b562 Chains: A, D
Molecule details ›
Chains: A, D
Length: 457 amino acids
Theoretical weight: 51.5 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41595 (Residues: 36-248, 314-405; Coverage: 63%)
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene names: HTR2B, cybC
Sequence domains:
Anti-5-HT2B Fab light chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 213 amino acids
Theoretical weight: 23.15 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins
Anti-5-HT2B Fab heavy chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 236 amino acids
Theoretical weight: 25.48 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ERM 2 x ERM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: P21
Unit cell:
a: 71.29Å b: 118.86Å c: 145.38Å
α: 90° β: 90.37° γ: 90°
R-values:
R R work R free
0.228 0.227 0.247
Expression system: Spodoptera frugiperda

Quick links

Citations

11 review citations

Structural insights into emergent signaling modes of G protein-coupled receptors.
Sutkeviciute et al. (2020)
10 more

17 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
16 more