› 5txf

X-ray diffraction
3.1Å resolution

Crystal structure of Lecithin:cholesterol acyltransferase (LCAT) in a closed conformation

Released:
DOI: 10.2210/pdb5txf/pdb
PDB entry 5txf coloured by chain, front view.
PDB entry 5txf coloured by chain, side view.
PDB entry 5txf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A retractable lid in lecithin:cholesterol acyltransferase provides a structural mechanism for activation by apolipoprotein A-I.
Manthei KA, Ahn J, Glukhova A, Yuan W, Larkin C, Manett TD, Chang L, Shayman JA, Axley MJ, Schwendeman A, Tesmer JJG
J Biol Chem 292 20313-20327 (2017)
PMID: 29030428

Function and Biology Details

Reactions catalysed: 
Phosphatidylcholine + a sterol = 1-acylglycerophosphocholine + a sterol ester
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl-sn-glycero-3-phosphocholine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Phosphatidylcholine-sterol acyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 422 amino acids
Theoretical weight: 47.96 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P04180 (Residues: 25-440; Coverage: 100%)
Gene name: LCAT
Sequence domains: Lecithin:cholesterol acyltransferase
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D, APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 95.887Å b: 123.532Å c: 114.778Å
α: 90° β: 96.19° γ: 90°
R-values:
R R work R free
0.25 0.25 0.267
Expression system: Homo sapiens

Quick links

Citations

2 review citations

Pathways and Mechanisms of Cellular Cholesterol Efflux-Insight From Imaging.
Juhl et al. (2022)
1 more

9 mentions without citation

A retractable lid in lecithin:cholesterol acyltransferase provides a structural mechanism for activation by apolipoprotein A-I.
Manthei et al. (2017)
8 more