› 5tyi

X-ray diffraction
2.15Å resolution

Grb7 SH2 with bicyclic peptide containing pY mimetic

Released:
DOI: 10.2210/pdb5tyi/pdb
PDB entry 5tyi coloured by chain, front view.
PDB entry 5tyi coloured by chain, side view.
PDB entry 5tyi coloured by chain, top view.
Source organisms:
Primary publication:
Discovery, Development, and Cellular Delivery of Potent and Selective Bicyclic Peptide Inhibitors of Grb7 Cancer Target.
Watson GM, Kulkarni K, Sang J, Ma X, Gunzburg MJ, Perlmutter P, Wilce MCJ, Wilce JA
J Med Chem 60 9349-9359 (2017)
PMID: 29083893

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero pentamer
Assembly name:
PDBe Complex ID:
PDB-CPX-166133 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Growth factor receptor-bound protein 7 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 120 amino acids
Theoretical weight: 13.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14451 (Residues: 415-532; Coverage: 22%)
Gene name: GRB7
Sequence domains: SH2 domain
Structure domains: SH2 domain
Peptide inhibitor Chains: L, M, N, P
Molecule details ›
Chains: L, M, N, P
Length: 9 amino acids
Theoretical weight: 1.19 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 45.07Å b: 107.61Å c: 48.012Å
α: 90° β: 101.38° γ: 90°
R-values:
R R work R free
0.209 0.207 0.246
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Grb7, a Critical Mediator of EGFR/ErbB Signaling, in Cancer Development and as a Potential Therapeutic Target.
Chu et al. (2019)
4 more