› 5u03

Electron Microscopy
6.1Å resolution

Cryo-EM structure of the human CTP synthase filament

Released:
DOI: 10.2210/pdb5u03/pdb
PDB entry 5u03 coloured by chain, front view.
PDB entry 5u03 coloured by chain, side view.
PDB entry 5u03 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human CTP synthase filament structure reveals the active enzyme conformation.
Lynch EM, Hicks DR, Shepherd M, Endrizzi JA, Maker A, Hansen JM, Barry RM, Gitai Z, Baldwin EP, Kollman JM
Nat Struct Mol Biol 24 507-514 (2017)
PMID: 28459447
Related structures: EMD-8474

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148183 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CTP synthase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 591 amino acids
Theoretical weight: 66.78 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P17812 (Residues: 1-591; Coverage: 100%)
Gene names: CTPS, CTPS1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 4 x ATP
Ligand UTP 4 x UTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.1Å
Relevant EMDB volumes: EMD-8474
Expression system: Saccharomyces cerevisiae

Quick links

Citations

14 review citations

Enzymatic Noncovalent Synthesis.
He et al. (2020)
13 more

7 mentions without citation

Infinite Assembly of Folded Proteins in Evolution, Disease, and Engineering.
Garcia-Seisdedos et al. (2019)
6 more