› 5u0j

X-ray diffraction
1.72Å resolution

C-terminal ankyrin repeats from human kidney-type glutaminase (KGA) - monoclinic crystal form

Released:
DOI: 10.2210/pdb5u0j/pdb
PDB entry 5u0j coloured by chain, front view.
PDB entry 5u0j coloured by chain, side view.
PDB entry 5u0j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The origin and evolution of human glutaminases and their atypical C-terminal ankyrin repeats.
Pasquali CC, Islam Z, Adamoski D, Ferreira IM, Righeto RD, Bettini J, Portugal RV, Yue WW, Gonzalez A, Dias SMG, Ambrosio ALB
J Biol Chem 292 11572-11585 (2017)
PMID: 28526749

Function and Biology Details

Reaction catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131796 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminase kidney isoform, mitochondrial 65 kDa chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 140 amino acids
Theoretical weight: 15.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O94925 (Residues: 551-669; Coverage: 18%)
Gene names: GLS, GLS1, KIAA0838
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

1 bound ligand:
Ligand NA 6 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 76.49Å b: 47.18Å c: 115.698Å
α: 90° β: 90.09° γ: 90°
R-values:
R R work R free
0.185 0.184 0.222
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.
Li et al. (2019)
4 more

1 mention without citation

The origin and evolution of human glutaminases and their atypical C-terminal ankyrin repeats.
Pasquali et al. (2017)