› 5u1f

Electron Microscopy
6.8Å resolution

Initial contact of HIV-1 Env with CD4: Cryo-EM structure of BG505 DS-SOSIP trimer in complex with CD4 and antibody PGT145

Released:
DOI: 10.2210/pdb5u1f/pdb
PDB entry 5u1f coloured by chain, front view.
PDB entry 5u1f coloured by chain, side view.
PDB entry 5u1f coloured by chain, top view.
Source organisms:
Primary publication:
Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer.
Liu Q, Acharya P, Dolan MA, Zhang P, Guzzo C, Lu J, Kwon A, Gururani D, Miao H, Bylund T, Chuang GY, Druz A, Zhou T, Rice WJ, Wigge C, Carragher B, Potter CS, Kwong PD, Lusso P
Nat Struct Mol Biol 24 370-378 (2017)
PMID: 28218750
Related structures: EMD-8427

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-208503 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Envelope glycoprotein gp160 Chains: A, C, D
Molecule details ›
Chains: A, C, D
Length: 501 amino acids
Theoretical weight: 55.88 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 29-502; Coverage: 55%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Transmembrane protein gp41 Chains: B, E, F
Molecule details ›
Chains: B, E, F
Length: 178 amino acids
Theoretical weight: 20.2 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 508-661; Coverage: 18%)
Gene name: env
Sequence domains: Retroviral envelope protein
PGT145 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 267 amino acids
Theoretical weight: 28.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
PGT145 light chain Chain: L
Molecule details ›
Chain: L
Length: 239 amino acids
Theoretical weight: 26.05 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
T-cell surface glycoprotein CD4 Chain: M
Molecule details ›
Chain: M
Length: 363 amino acids
Theoretical weight: 40.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01730 (Residues: 26-388; Coverage: 84%)
Gene name: CD4
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TYS 2 x TYS

Experiments and Validation Details

Entry percentile scores
Resolution: 6.8Å
Relevant EMDB volumes: EMD-8427
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Cryo-EM in drug discovery: achievements, limitations and prospects.
Renaud et al. (2018)
6 more

23 mentions without citation

Molecular Mechanism of HIV-1 Entry.
Chen B. (2019)
22 more