› 5u5p

X-ray diffraction
2.17Å resolution

Crystal Structure and X-ray Diffraction Data Collection of Importin-alpha from Mus Musculus Complexed with a MLH1 NLS Peptide

Released:
DOI: 10.2210/pdb5u5p/pdb
PDB entry 5u5p coloured by chain, front view.
PDB entry 5u5p coloured by chain, side view.
PDB entry 5u5p coloured by chain, top view.
Source organisms:
Primary publication:
DNA mismatch repair proteins MLH1 and PMS2 can be imported to the nucleus by a classical nuclear import pathway.
de Barros AC, Takeda AAS, Dreyer TR, Velazquez-Campoy A, Kobe B, Fontes MRM
Biochimie 146 87-96 (2018)
PMID: 29175432

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154161 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA mismatch repair protein Mlh1 Chains: B, C
Molecule details ›
Chains: B, C
Length: 10 amino acids
Theoretical weight: 1.3 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P40692 (Residues: 467-476; Coverage: 1%)
Gene names: COCA2, MLH1
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 55.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 77.215Å b: 89.071Å c: 99.139Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.211
Expression systems:
  • Not provided
  • Escherichia coli

Quick links

Citations

1 review citation

Genetic Polymorphisms of DNA Repair Pathways in Sporadic Colorectal Carcinogenesis.
Liu et al. (2019)
5 other articles

1 mention without citation

Comparative study of the interactions between fungal transcription factor nuclear localization sequences with mammalian and fungal importin-alpha.
Bernardes et al. (2020)