› 5u66

X-ray diffraction
1.7Å resolution

Modified single helix from the B-domain of protein A bound to IgG1 Fc

Released:
DOI: 10.2210/pdb5u66/pdb
PDB entry 5u66 coloured by chain, front view.
PDB entry 5u66 coloured by chain, side view.
PDB entry 5u66 coloured by chain, top view.
Source organisms:
Primary publication:
3-2-1: Structural insights from stepwise shrinkage of a three-helix Fc-binding domain to a single helix.
Ultsch M, Braisted A, Maun HR, Eigenbrot C
Protein Eng Des Sel 30 619-625 (2017)
PMID: 28475752

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134873 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
STAPLED PEPTIDE FROM DOMAIN B OF PROTEIN A Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 2.04 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided
Immunoglobulin heavy constant gamma 1 Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.58 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01857 (Residues: 120-326; Coverage: 52%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1, RIGAKU RU300
Spacegroup: C2221
Unit cell:
a: 49.165Å b: 126.812Å c: 91.927Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.157 0.227
Expression systems:
  • Not provided
  • Cricetulus griseus

Quick links

Citations

8 citation in other articles

Population-wide diversity and stability of serum antibody epitope repertoires against human microbiota.
Vogl et al. (2021)
7 more

3 mentions without citation

Stapled Peptides Inhibitors: A New Window for Target Drug Discovery.
Ali et al. (2019)
2 more