› 5u74

X-ray diffraction
3.34Å resolution

Structure of human Niemann-Pick C1 protein

Released:
DOI: 10.2210/pdb5u74/pdb
PDB entry 5u74 coloured by chain, front view.
PDB entry 5u74 coloured by chain, side view.
PDB entry 5u74 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
3.3 Å structure of Niemann-Pick C1 protein reveals insights into the function of the C-terminal luminal domain in cholesterol transport.
Li X, Lu F, Trinh MN, Schmiege P, Seemann J, Wang J, Blobel G
Proc Natl Acad Sci U S A 114 9116-9121 (2017)
PMID: 28784760

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
NPC intracellular cholesterol transporter 1 Chain: A
Molecule details ›
Chain: A
Length: 1278 amino acids
Theoretical weight: 142.25 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O15118 (Residues: 1-1278; Coverage: 100%)
Gene name: NPC1
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 8 x NAG
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2221
Unit cell:
a: 174.87Å b: 222.115Å c: 107.851Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.246 0.299
Expression system: Homo sapiens

Quick links

Citations

9 review citations

NPC intracellular cholesterol transporter 1 (NPC1)-mediated cholesterol export from lysosomes.
Pfeffer SR. (2019)
8 more

14 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
13 more