› 5u78

X-ray diffraction
1.98Å resolution

Crystal structure of ORP8 PH domain in P1211 space group

Released:
DOI: 10.2210/pdb5u78/pdb
PDB entry 5u78 coloured by chain, front view.
PDB entry 5u78 coloured by chain, side view.
PDB entry 5u78 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
ORP5 and ORP8 bind phosphatidylinositol-4, 5-biphosphate (PtdIns(4,5)P 2) and regulate its level at the plasma membrane.
Ghai R, Du X, Wang H, Dong J, Ferguson C, Brown AJ, Parton RG, Wu JW, Yang H
Nat Commun 8 757 (2017)
PMID: 28970484

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxysterol-binding protein-related protein 8 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 123 amino acids
Theoretical weight: 13.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BZF1 (Residues: 149-265; Coverage: 13%)
Gene names: KIAA1451, ORP8, OSBP10, OSBPL8
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 52.17Å b: 66.35Å c: 79.549Å
α: 90° β: 94.67° γ: 90°
R-values:
R R work R free
0.224 0.223 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

38 review citations

The functional universe of membrane contact sites.
Prinz et al. (2020)
37 more

1 mention without citation

ORP5 and ORP8 bind phosphatidylinositol-4, 5-biphosphate (PtdIns(4,5)P 2) and regulate its level at the plasma membrane.
Ghai et al. (2017)