› 5u8q

X-ray diffraction
3.27Å resolution

Structure of the ectodomain of the human Type 1 insulin-like growth factor receptor in complex with IGF-I

Released:
DOI: 10.2210/pdb5u8q/pdb
PDB entry 5u8q coloured by chain, front view.
PDB entry 5u8q coloured by chain, side view.
PDB entry 5u8q coloured by chain, top view.
Source organisms:
Primary publication:
How ligand binds to the type 1 insulin-like growth factor receptor.
Xu Y, Kong GK, Menting JG, Margetts MB, Delaine CA, Jenkin LM, Kiselyov VV, De Meyts P, Forbes BE, Lawrence MC
Nat Commun 9 821 (2018)
PMID: 29483580

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-230795 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Insulin-like growth factor 1 receptor alpha chain Chain: A
Molecule details ›
Chain: A
Length: 885 amino acids
Theoretical weight: 101.09 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P08069 (Residues: 31-935; Coverage: 66%)
Gene name: IGF1R
Sequence domains:
Insulin-like growth factor I Chain: B
Molecule details ›
Chain: B
Length: 70 amino acids
Theoretical weight: 7.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05019 (Residues: 49-118; Coverage: 40%)
Gene names: IBP1, IGF1
Sequence domains: Insulin/IGF/Relaxin family
Structure domains: Insulin-like
Fv 24-60 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 126 amino acids
Theoretical weight: 13.95 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Fv 24-60 light chain Chain: L
Molecule details ›
Chain: L
Length: 108 amino acids
Theoretical weight: 11.73 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
3 bound ligands:
Ligand SO4 2 x SO4
Ligand MLT 1 x MLT
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21212
Unit cell:
a: 88.69Å b: 197.66Å c: 117.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.26 0.303
Expression systems:
  • Cricetulus griseus
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

17 review citations

Therapeutic Targeting of the IGF Axis.
Osher et al. (2019)
16 more

9 mentions without citation

Insulin and epidermal growth factor receptor family members share parallel activation mechanisms.
Ferguson et al. (2020)
8 more