› 5uan

X-ray diffraction
3.51Å resolution

Crystal structure of multi-domain RAR-beta-RXR-alpha heterodimer on DNA

Released:
DOI: 10.2210/pdb5uan/pdb
PDB entry 5uan coloured by chain, front view.
PDB entry 5uan coloured by chain, side view.
PDB entry 5uan coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The quaternary architecture of RARβ-RXRα heterodimer facilitates domain-domain signal transmission.
Chandra V, Wu D, Li S, Potluri N, Kim Y, Rastinejad F
Nat Commun 8 868 (2017)
PMID: 29021580

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-119205 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Retinoic acid receptor RXR-alpha Chain: A
Molecule details ›
Chain: A
Length: 365 amino acids
Theoretical weight: 41.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19793 (Residues: 98-462; Coverage: 79%)
Gene names: NR2B1, RXRA
Sequence domains:
Retinoic acid receptor beta Chain: B
Molecule details ›
Chain: B
Length: 397 amino acids
Theoretical weight: 44.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10826 (Residues: 80-455; Coverage: 83%)
Gene names: HAP, NR1B2, RARB
Sequence domains:
Structure domains:
Nuclear receptor coactivator 2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 amino acids
Theoretical weight: 1.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 687-696; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2
DNA (5'-D(*CP*TP*AP*GP*GP*TP*CP*AP*AP*AP*GP*GP*TP*CP*AP*GP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 17 nucleotides
Theoretical weight: 5.24 KDa
DNA (5'-D(*GP*CP*TP*GP*AP*CP*CP*TP*TP*TP*GP*AP*CP*CP*TP*AP*G)-3') Chain: F
Molecule details ›
Chain: F
Length: 17 nucleotides
Theoretical weight: 5.18 KDa

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand 9CR 1 x 9CR
Ligand REA 1 x REA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 52.47Å b: 77.403Å c: 112.113Å
α: 90° β: 90.37° γ: 90°
R-values:
R R work R free
0.222 0.221 0.27
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Nuclear receptor crosstalk - defining the mechanisms for therapeutic innovation.
De Bosscher et al. (2020)
6 more

5 mentions without citation

Full-length nuclear receptor allosteric regulation.
Choi et al. (2023)
4 more