› 5ucb

X-ray diffraction
1.52Å resolution

Structure of antigen-Fab complex with engineered switch residue region.

Released:
DOI: 10.2210/pdb5ucb/pdb
PDB entry 5ucb coloured by chain, front view.
PDB entry 5ucb coloured by chain, side view.
PDB entry 5ucb coloured by chain, top view.
Source organisms:
Entry authors: Bailey LJ, Kossiakoff AA, Chaperone-Enabled Studies of Epigenetic Regulation Enzymes (CEBS)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211068 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 217 amino acids
Theoretical weight: 23.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 210 amino acids
Theoretical weight: 22.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Histone chaperone ASF1 Chain: B
Molecule details ›
Chain: B
Length: 153 amino acids
Theoretical weight: 17.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32447 (Residues: 2-154; Coverage: 55%)
Gene names: ASF1, CIA1, J0755, YJL115W
Sequence domains: ASF1 like histone chaperone
Structure domains: Histone chaperone ASF1-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P1
Unit cell:
a: 45.378Å b: 49.18Å c: 84.707Å
α: 106.29° β: 95.71° γ: 100.13°
R-values:
R R work R free
0.14 0.138 0.169
Expression system: Escherichia coli

Quick links