› 5ue5

Solution NMR

proMMP-7 with heparin octasaccharide bound to the catalytic domain

Released:
DOI: 10.2210/pdb5ue5/pdb
PDB entry 5ue5 coloured by chain, ensemble of 16 models, front view.
PDB entry 5ue5 coloured by chain, ensemble of 16 models, side view.
PDB entry 5ue5 coloured by chain, ensemble of 16 models, top view.
Source organism: Homo sapiens
Primary publication:
Glycan Activation of a Sheddase: Electrostatic Recognition between Heparin and proMMP-7.
Fulcher YG, Prior SH, Masuko S, Li L, Pu D, Zhang F, Linhardt RJ, Van Doren SR
Structure 25 1100-1110.e5 (2017)
PMID: 28648610

Function and Biology Details

Reaction catalysed: 
Cleavage of 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in B chain of insulin. No action on collagen types I, II, IV, V. Cleaves gelatin chain alpha-2(I) > alpha-1(I).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Matrilysin Chain: A
Molecule details ›
Chain: A
Length: 247 amino acids
Theoretical weight: 27.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09237 (Residues: 21-267; Coverage: 99%)
Gene names: MMP7, MPSL1, PUMP1
Sequence domains:
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

Carbohydrate polymer : NEW Components: SGN, IDS
Carbohydrate polymer
2 bound ligands:
Ligand CA 2 x CA
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 85%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

A perspective on the PDB's impact on the field of glycobiology.
Prestegard JH. (2021)
2 more

2 mentions without citation

MMP-7 marks severe pancreatic cancer and alters tumor cell signaling by proteolytic release of ectodomains.
Van Doren SR. (2022)
1 more