› 5uek

X-ray diffraction
1.7Å resolution

Structure of antigen-Fab 12E complex with Histone chaperone ASF1

Released:
DOI: 10.2210/pdb5uek/pdb
PDB entry 5uek coloured by chain, front view.
PDB entry 5uek coloured by chain, side view.
PDB entry 5uek coloured by chain, top view.
Source organisms:
Entry authors: Bailey LJ, Kossiakoff AA

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211070 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Histone chaperone ASF1 Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.43 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32447 (Residues: 2-154; Coverage: 55%)
Gene names: ASF1, CIA1, J0755, YJL115W
Sequence domains: ASF1 like histone chaperone
Structure domains: Histone chaperone ASF1-like
Fab 12E Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 227 amino acids
Theoretical weight: 24.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab 12E Light Chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 43.175Å b: 142.932Å c: 49.401Å
α: 90° β: 98.8° γ: 90°
R-values:
R R work R free
0.178 0.176 0.212
Expression system: Escherichia coli

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