› 5ufq

X-ray diffraction
2.2Å resolution

K-RasG12D(GNP)/R11.1.6 complex

Released:
DOI: 10.2210/pdb5ufq/pdb
PDB entry 5ufq coloured by chain, front view.
PDB entry 5ufq coloured by chain, side view.
PDB entry 5ufq coloured by chain, top view.
Source organisms:
Primary publication:
An engineered protein antagonist of K-Ras/B-Raf interaction.
Kauke MJ, Traxlmayr MW, Parker JA, Kiefer JD, Knihtila R, McGee J, Verdine G, Mattos C, Wittrup KD
Sci Rep 7 5831 (2017)
PMID: 28724936

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134006 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTPase KRas, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 18.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-166; Coverage: 88%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
R11.1.6 Chains: C, D
Molecule details ›
Chains: C, D
Length: 61 amino acids
Theoretical weight: 7.26 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

5 bound ligands:
Ligand GNP 2 x GNP
Ligand MG 2 x MG
Ligand CL 4 x CL
Ligand CA 2 x CA
Ligand CD 2 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 119.717Å b: 42.707Å c: 100.235Å
α: 90° β: 113.33° γ: 90°
R-values:
R R work R free
0.206 0.2 0.263
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The current understanding of KRAS protein structure and dynamics.
Pantsar T. (2020)
10 more

9 mentions without citation

Direct inhibition of RAS: Quest for the Holy Grail?
Spencer-Smith et al. (2019)
8 more