› 5ulm

X-ray diffraction
2.1Å resolution

Structure of the ASK1 central regulatory region

Released:
DOI: 10.2210/pdb5ulm/pdb
PDB entry 5ulm coloured by chain, front view.
PDB entry 5ulm coloured by chain, side view.
PDB entry 5ulm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of autoregulatory scaffolding by apoptosis signal-regulating kinase 1.
Weijman JF, Kumar A, Jamieson SA, King CM, Caradoc-Davies TT, Ledgerwood EC, Murphy JM, Mace PD
Proc Natl Acad Sci U S A 114 E2096-E2105 (2017)
PMID: 28242696

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189242 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase kinase kinase 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 45.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99683 (Residues: 269-658; Coverage: 28%)
Gene names: ASK1, MAP3K5, MAPKKK5, MEKK5
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 74.234Å b: 57.117Å c: 103.575Å
α: 90° β: 104.87° γ: 90°
R-values:
R R work R free
0.235 0.233 0.267
Expression system: Escherichia coli

Quick links

Citations

8 review citations

New insights into the role and mechanism of c-Jun-N-terminal kinase signaling in the pathobiology of liver diseases.
Win et al. (2018)
7 more

3 mentions without citation

Structural Insights Support Targeting ASK1 Kinase for Therapeutic Interventions.
Obsilova et al. (2021)
2 more