› 5umu

X-ray diffraction
1.9Å resolution

Crystal structure of the middle double PH domain of human FACT complex subunit SPT16

Released:
DOI: 10.2210/pdb5umu/pdb
PDB entry 5umu coloured by chain, front view.
PDB entry 5umu coloured by chain, side view.
PDB entry 5umu coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Hu Q, Thompson JR, Heroux A, Su D, Botuyan MV, Mer G

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FACT complex subunit SPT16 Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 33.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5B9 (Residues: 649-926; Coverage: 27%)
Gene names: FACT140, FACTP140, SUPT16H
Sequence domains: Histone chaperone Rttp106-like, middle domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FMT 25 x FMT
Ligand ACT 5 x ACT
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 50.221Å b: 99.819Å c: 120.314Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.191
Expression system: Escherichia coli BL21(DE3)

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