5und

X-ray diffraction
2.55Å resolution

Crystal Structure of CTCF(ZnF 4-10) With 28-mer DNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-119226 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional repressor CTCF Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 24.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49711 (Residues: 348-547; Coverage: 28%)
Gene name: CTCF
Sequence domains: Zinc finger, C2H2 type
DNA (26-MER) Chains: C, E
Molecule details ›
Chains: C, E
Length: 28 nucleotides
Theoretical weight: 8.53 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (26-MER) Chains: D, F
Molecule details ›
Chains: D, F
Length: 28 nucleotides
Theoretical weight: 8.69 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 75.072Å b: 73.825Å c: 93.289Å
α: 90° β: 91.38° γ: 90°
R-values:
R R work R free
0.212 0.211 0.237
Expression systems:
  • Escherichia coli
  • Not provided