› 5uoz

X-ray diffraction
1.17Å resolution

Insulin with proline analog FyP at position B28 in the T2 state

Released:
DOI: 10.2210/pdb5uoz/pdb
PDB entry 5uoz coloured by chain, front view.
PDB entry 5uoz coloured by chain, side view.
PDB entry 5uoz coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Lieblich SA, Fang KY, Tirrell DA

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134210 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
1 modified residue:
Ligand FP9 1 x FP9

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I213
Unit cell:
a: 78.094Å b: 78.094Å c: 78.094Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.141 0.155
Expression system: Escherichia coli

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