› 5upp

X-ray diffraction
1.8Å resolution

Crystal structure of human fumarate hydratase

Released:
DOI: 10.2210/pdb5upp/pdb
PDB entry 5upp coloured by chain, front view.
PDB entry 5upp coloured by chain, side view.
PDB entry 5upp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural, biochemical and biophysical characterization of recombinant human fumarate hydratase.
Ajalla Aleixo MA, Rangel VL, Rustiguel JK, de Pádua RAP, Nonato MC
FEBS J 286 1925-1940 (2019)
PMID: 30761759

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139860 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 466 amino acids
Theoretical weight: 50.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07954 (Residues: 45-510; Coverage: 91%)
Gene name: FH
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 125.434Å b: 148.006Å c: 129.761Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 citation in other articles

A human pluripotent stem cell model for the analysis of metabolic dysfunction in hepatic steatosis.
Sinton et al. (2021)
7 more

4 mentions without citation

An evolving view of complex II-noncanonical complexes, megacomplexes, respiration, signaling, and beyond.
Iverson et al. (2023)
3 more