› 5uqa

X-ray diffraction
1.31Å resolution

Insulin with proline analog FzP at position B28 in the R6 state

Released:
DOI: 10.2210/pdb5uqa/pdb
PDB entry 5uqa coloured by chain, front view.
PDB entry 5uqa coloured by chain, side view.
PDB entry 5uqa coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Lieblich SA, Fang KY, Tirrell DA

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134235 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 30 amino acids
Theoretical weight: 3.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

6 bound ligands:
Ligand IPH 6 x IPH
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand NA 6 x NA
Ligand GOL 1 x GOL
Ligand ACN 1 x ACN
1 modified residue:
Ligand 4FB 6 x 4FB

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 46.936Å b: 61.258Å c: 60.39Å
α: 90° β: 111.694° γ: 90°
R-values:
R R work R free
0.154 0.152 0.182
Expression system: Escherichia coli

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