› 5urj

X-ray diffraction
2.75Å resolution

Crystal structure of human BRR2 in complex with T-3905516

Released:
DOI: 10.2210/pdb5urj/pdb
PDB entry 5urj coloured by chain, front view.
PDB entry 5urj coloured by chain, side view.
PDB entry 5urj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Discovery of Allosteric Inhibitors Targeting the Spliceosomal RNA Helicase Brr2.
Iwatani-Yoshihara M, Ito M, Klein MG, Yamamoto T, Yonemori K, Tanaka T, Miwa M, Morishita D, Endo S, Tjhen R, Qin L, Nakanishi A, Maezaki H, Kawamoto T
J Med Chem 60 5759-5771 (2017)
PMID: 28586220

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131090 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
U5 small nuclear ribonucleoprotein 200 kDa helicase Chain: A
Molecule details ›
Chain: A
Length: 1738 amino acids
Theoretical weight: 198.58 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O75643 (Residues: 395-2129; Coverage: 81%)
Gene names: ASCC3L1, BRR2, HELIC2, KIAA0788, SNRNP200
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand 8LS 1 x 8LS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 144.909Å b: 153.954Å c: 142.474Å
α: 90° β: 120.5° γ: 90°
R-values:
R R work R free
0.24 0.237 0.286
Expression system: Spodoptera frugiperda

Quick links

Citations

12 review citations

Roles and mechanisms of alternative splicing in cancer - implications for care.
Bonnal et al. (2020)
11 more