› 5urn

Solution NMR

NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and the transactivation domain 1 of p65

Released:
DOI: 10.2210/pdb5urn/pdb
PDB entry 5urn coloured by chain, ensemble of 20 models, front view.
PDB entry 5urn coloured by chain, ensemble of 20 models, side view.
PDB entry 5urn coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Structural characterization of interactions between transactivation domain 1 of the p65 subunit of NF-κB and transcription regulatory factors.
Lecoq L, Raiola L, Chabot PR, Cyr N, Arseneault G, Legault P, Omichinski JG
Nucleic Acids Res 45 5564-5576 (2017)
PMID: 28334776

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152446 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
General transcription and DNA repair factor IIH subunit TFB1 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 12.9 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32776 (Residues: 2-115; Coverage: 18%)
Gene names: D9740.3, TFB1, YDR311W
Sequence domains: TFIIH p62 subunit, N-terminal domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Transcription factor p65 Chain: B
Molecule details ›
Chain: B
Length: 33 amino acids
Theoretical weight: 3.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04206 (Residues: 521-551; Coverage: 6%)
Gene names: NFKB3, RELA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 94%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Small Molecule NF-κB Pathway Inhibitors in Clinic.
Ramadass et al. (2020)
3 more

3 mentions without citation

Genome reading by the NF-κB transcription factors.
Mulero et al. (2019)
2 more