› 5ury

X-ray diffraction
2.1Å resolution

Crystal structure of Frizzled 5 CRD in complex with PAM

Released:
DOI: 10.2210/pdb5ury/pdb
PDB entry 5ury coloured by chain, front view.
PDB entry 5ury coloured by chain, side view.
PDB entry 5ury coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unsaturated fatty acyl recognition by Frizzled receptors mediates dimerization upon Wnt ligand binding.
Nile AH, Mukund S, Stanger K, Wang W, Hannoush RN
Proc Natl Acad Sci U S A 114 4147-4152 (2017)
PMID: 28377511

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171704 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Frizzled-5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 16.77 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q13467 (Residues: 28-155; Coverage: 23%)
Gene names: C2orf31, FZD5
Sequence domains: Fz domain
Structure domains: Frizzled cysteine-rich domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
1 bound ligand:
Ligand PAM 2 x PAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P3121
Unit cell:
a: 123.412Å b: 123.412Å c: 46.951Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.17 0.206
Expression system: Trichoplusia ni

Quick links

Citations

15 review citations

WNT signalling in prostate cancer.
Murillo-Garzón et al. (2017)
14 more

6 mentions without citation

Assembly and architecture of the Wnt/β-catenin signalosome at the membrane.
DeBruine et al. (2017)
5 more