› 5uum

X-ray diffraction
2.35Å resolution

Human Mcl-1 in complex with a Bfl-1-specific selected peptide

Released:
DOI: 10.2210/pdb5uum/pdb
PDB entry 5uum coloured by chain, front view.
PDB entry 5uum coloured by chain, side view.
PDB entry 5uum coloured by chain, top view.
Source organisms:
Primary publication:
Epistatic mutations in PUMA BH3 drive an alternate binding mode to potently and selectively inhibit anti-apoptotic Bfl-1.
Jenson JM, Ryan JA, Grant RA, Letai A, Keating AE
Elife 6 (2017)
PMID: 28594323

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166182 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Induced myeloid leukemia cell differentiation protein Mcl-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 156 amino acids
Theoretical weight: 17.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07820 (Residues: 172-325; Coverage: 44%)
Gene names: BCL2L3, MCL1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Structure domains: Blc2-like
Bfl-1 specific peptide FS2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 25 amino acids
Theoretical weight: 2.65 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 132.617Å b: 62.76Å c: 48.788Å
α: 90° β: 98.14° γ: 90°
R-values:
R R work R free
0.22 0.216 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Molecular evolution of peptides by yeast surface display technology.
Linciano et al. (2019)
1 more

1 mention without citation

Tertiary Structural Motif Sequence Statistics Enable Facile Prediction and Design of Peptides that Bind Anti-apoptotic Bfl-1 and Mcl-1.
Frappier et al. (2019)