› 5uut

X-ray diffraction
2.25Å resolution

N-myristoyltransferase 1 (NMT) bound to myristoyl-CoA

Released:
DOI: 10.2210/pdb5uut/pdb
PDB entry 5uut coloured by chain, front view.
PDB entry 5uut coloured by chain, side view.
PDB entry 5uut coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Blocking Myristoylation of Src Inhibits Its Kinase Activity and Suppresses Prostate Cancer Progression.
Kim S, Alsaidan OA, Goodwin O, Li Q, Sulejmani E, Han Z, Bai A, Albers T, Beharry Z, Zheng YG, Norris JS, Szulc ZM, Bielawska A, Lebedyeva I, Pegan SD, Cai H
Cancer Res 77 6950-6962 (2017)
PMID: 29038344

Function and Biology Details

Reaction catalysed: 
Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151725 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycylpeptide N-tetradecanoyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 45.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30419 (Residues: 109-496; Coverage: 78%)
Gene names: NMT, NMT1
Sequence domains:
Structure domains: Aminopeptidase

Ligands and Environments


Cofactor: Ligand MYA 2 x MYA
1 bound ligand:
Ligand CIT 2 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 78.778Å b: 177.582Å c: 58.566Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.221
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Src Family Kinases as Therapeutic Targets in Advanced Solid Tumors: What We Have Learned so Far.
Martellucci et al. (2020)
17 more

2 mentions without citation

Encapsulating Cas9 into extracellular vesicles by protein myristoylation.
Whitley et al. (2022)
1 more