5uwq

X-ray diffraction
2.28Å resolution

Crystal Structure of CDC7 NES Peptide in complex with CRM1-Ran-RanBP1

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-126242 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
GTP-binding nuclear protein Ran Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62826 (Residues: 1-216; Coverage: 100%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Ran-specific GTPase-activating protein 1 Chain: B
Molecule details ›
Chain: B
Length: 143 amino acids
Theoretical weight: 16.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41920 (Residues: 62-201; Coverage: 70%)
Gene names: CST20, HTN1, SFO1, YD8119.08, YDR002W, YRB1
Sequence domains: RanBP1 domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Exportin-1 Chain: C
Molecule details ›
Chain: C
Length: 1024 amino acids
Theoretical weight: 117.46 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30822 (Residues: 1-1058; Coverage: 94%)
Gene names: CRM1, G8514, KAP124, XPO1, YGR218W
Sequence domains:
Cell division cycle 7-related protein kinase Chain: D
Molecule details ›
Chain: D
Length: 22 amino acids
Theoretical weight: 2.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00311 (Residues: 456-473; Coverage: 3%)
Gene names: CDC7, CDC7L1

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 106.392Å b: 106.392Å c: 304.567Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.225
Expression system: Escherichia coli BL21(DE3)