› 5uzr

X-ray diffraction
2.3Å resolution

Crystal structure of citrate synthase from homo sapiens

Released:
DOI: 10.2210/pdb5uzr/pdb
PDB entry 5uzr coloured by chain, front view.
PDB entry 5uzr coloured by chain, side view.
PDB entry 5uzr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Comparative studies of Aspergillus fumigatus 2-methylcitrate synthase and human citrate synthase.
Schlachter CR, Klapper V, Radford T, Chruszcz M
Biol Chem 400 1567-1581 (2019)
PMID: 31141475

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130940 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate synthase, mitochondrial Chains: A, D
Molecule details ›
Chains: A, D
Length: 462 amino acids
Theoretical weight: 51.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75390 (Residues: 28-466; Coverage: 94%)
Gene name: CS
Sequence domains: Citrate synthase, C-terminal domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 58.177Å b: 74.676Å c: 60.652Å
α: 62.78° β: 93.85° γ: 80.3°
R-values:
R R work R free
0.172 0.17 0.215
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Pathogenicity and virulence of Aspergillus fumigatus.
Earle et al. (2023)
2 more

1 mention without citation

Structural and biochemical characterization of mitochondrial citrate synthase 4 from Arabidopsis thaliana.
Nishio et al. (2020)