› 5v0l

X-ray diffraction
4Å resolution

Crystal structure of the AHR-ARNT heterodimer in complex with the DRE

Released:
DOI: 10.2210/pdb5v0l/pdb
PDB entry 5v0l coloured by chain, front view.
PDB entry 5v0l coloured by chain, side view.
PDB entry 5v0l coloured by chain, top view.
Source organisms:
Primary publication:
Structural hierarchy controlling dimerization and target DNA recognition in the AHR transcriptional complex.
Seok SH, Lee W, Jiang L, Molugu K, Zheng A, Li Y, Park S, Bradfield CA, Xing Y
Proc Natl Acad Sci U S A 114 5431-5436 (2017)
PMID: 28396409

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119255 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Aryl hydrocarbon receptor nuclear translocator Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 31.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P27540 (Residues: 70-346; Coverage: 35%)
Gene names: ARNT, BHLHE2
Sequence domains:
Aryl hydrocarbon receptor Chain: B
Molecule details ›
Chain: B
Length: 241 amino acids
Theoretical weight: 27.15 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P30561 (Residues: 29-267; Coverage: 28%)
Gene name: Ahr
Sequence domains:
DNA (5'-D(P*GP*GP*AP*TP*TP*GP*CP*GP*TP*GP*AP*GP*AP*AP*CP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 17 nucleotides
Theoretical weight: 5.31 KDa
DNA (5'-D(P*AP*GP*TP*TP*CP*TP*CP*AP*CP*GP*CP*AP*AP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 14 nucleotides
Theoretical weight: 4.24 KDa

Ligands and Environments

2 bound ligands:
Ligand PEG 2 x PEG
Ligand CIT 2 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 78.199Å b: 64.364Å c: 157.725Å
α: 90° β: 100.14° γ: 90°
R-values:
R R work R free
0.287 0.285 0.323
Expression system: Escherichia coli

Quick links

Citations

16 review citations

The tryptophan derivative 6-formylindolo[3,2-b]carbazole, FICZ, a dynamic mediator of endogenous aryl hydrocarbon receptor signaling, balances cell growth and differentiation.
Rannug et al. (2018)
15 more

9 mentions without citation

bHLH-PAS Proteins: Their Structure and Intrinsic Disorder.
Kolonko et al. (2019)
8 more