› 5v5o

X-ray diffraction
2.24Å resolution

Structure of NLS2K of influenza A virus nucleoprotein bound to importin alpha

Released:
DOI: 10.2210/pdb5v5o/pdb
PDB entry 5v5o coloured by chain, front view.
PDB entry 5v5o coloured by chain, side view.
PDB entry 5v5o coloured by chain, top view.
Source organisms:
Primary publication:
Synergy of two low-affinity NLSs determines the high avidity of influenza A virus nucleoprotein NP for human importin α isoforms.
Wu W, Sankhala RS, Florio TJ, Zhou L, Nguyen NLT, Lokareddy RK, Cingolani G, Panté N
Sci Rep 7 11381 (2017)
PMID: 28900157

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137054 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 528 amino acids
Theoretical weight: 57.86 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52293 (Residues: 2-529; Coverage: 100%)
Gene names: Kpna2, Rch1
Sequence domains:
Nucleoprotein Chains: A, B
Molecule details ›
Chains: A, B
Length: 19 amino acids
Theoretical weight: 2.37 KDa
Source organism: Influenza A virus
Expression system: Not provided
UniProt:
  • Canonical: P03466 (Residues: 198-216; Coverage: 4%)
Gene name: NP

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 78.165Å b: 90.893Å c: 97.086Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.206
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Zoonotic Potential of Influenza A Viruses: A Comprehensive Overview.
Mostafa et al. (2018)
2 more

3 mentions without citation

Synergy of two low-affinity NLSs determines the high avidity of influenza A virus nucleoprotein NP for human importin α isoforms.
Wu et al. (2017)
2 more