5v6y

X-ray diffraction
2.8Å resolution

Crystal structure of the human CLR:RAMP1 extracellular domain heterodimer with bound high-affinity and altered selectivity adrenomedullin variant

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-199561 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Calcitonin gene-related peptide type 1 receptor; Maltose/maltodextrin-binding periplasmic protein; Receptor activity-modifying protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 593 amino acids
Theoretical weight: 66.3 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 25-392; Coverage: 99%)
  • Canonical: O60894 (Residues: 24-111; Coverage: 70%)
  • Canonical: Q16602 (Residues: 29-144; Coverage: 26%)
Gene names: CALCRL, CGRPR, JW3994, RAMP1, b4034, malE
Sequence domains:
Adrenomedullin Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 17 amino acids
Theoretical weight: 1.9 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P35318 (Residues: 131-146; Coverage: 10%)
Gene names: ADM, AM

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 195.496Å b: 69.436Å c: 200Å
α: 90° β: 99.43° γ: 90°
R-values:
R R work R free
0.213 0.212 0.244
Expression systems:
  • Escherichia coli
  • Not provided