› 5v7x

X-ray diffraction
3.14Å resolution

Crystal Structure of Myosin 1b residues 1-728 with bound sulfate and Calmodulin

Released:
DOI: 10.2210/pdb5v7x/pdb
PDB entry 5v7x coloured by chain, front view.
PDB entry 5v7x coloured by chain, side view.
PDB entry 5v7x coloured by chain, top view.
Source organisms:
Primary publication:
High-resolution cryo-EM structures of actin-bound myosin states reveal the mechanism of myosin force sensing.
Mentes A, Huehn A, Liu X, Zwolak A, Dominguez R, Shuman H, Ostap EM, Sindelar CV
Proc Natl Acad Sci U S A 115 1292-1297 (2018)
PMID: 29358376

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143940 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Unconventional myosin-Ib Chain: A
Molecule details ›
Chain: A
Length: 752 amino acids
Theoretical weight: 86.95 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q05096 (Residues: 1-728; Coverage: 64%)
Gene names: Myo1a, Myo1b, Myr1
Sequence domains:
Calmodulin-1 Chain: B
Molecule details ›
Chain: B
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 109.765Å b: 48.73Å c: 118.512Å
α: 90° β: 89.95° γ: 90°
R-values:
R R work R free
0.193 0.188 0.202
Expression system: Spodoptera frugiperda

Quick links

Citations

12 review citations

Towards a structural understanding of the remodeling of the actin cytoskeleton.
Merino et al. (2020)
11 more

4 mentions without citation

High-resolution cryo-EM structures of actin-bound myosin states reveal the mechanism of myosin force sensing.
Mentes et al. (2018)
3 more