› 5v8t

X-ray diffraction
2.1Å resolution

Crystal structure of SMT fusion Peptidyl-prolyl cis-trans isomerase from Burkholderia pseudomallei complexed with SF354

Released:
DOI: 10.2210/pdb5v8t/pdb
PDB entry 5v8t coloured by chain, front view.
PDB entry 5v8t coloured by chain, side view.
PDB entry 5v8t coloured by chain, top view.
Source organisms:
Entry authors: Lorimer DD, Dranow DM, Seufert F, Abendroth J, Holzgrabe U

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171336 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidyl-prolyl cis-trans isomerase; Ubiquitin-like protein SMT3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 208 amino acids
Theoretical weight: 22.87 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12306 (Residues: 13-97; Coverage: 84%)
  • Canonical: Q63J95 (Residues: 2-113; Coverage: 99%)
Gene names: BPSS1823, D9719.15, SMT3, YDR510W, fbp
Sequence domains:
Structure domains: Chitinase A; domain 3

Ligands and Environments

3 bound ligands:
Ligand 8ZV 2 x 8ZV
Ligand FMT 1 x FMT
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 60.64Å b: 32.5Å c: 99.35Å
α: 90° β: 96.73° γ: 90°
R-values:
R R work R free
0.187 0.184 0.221
Expression system: Escherichia coli BL21(DE3)

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