› 5v9i

X-ray diffraction
1.74Å resolution

Crystal structure of catalytic domain of G9a with MS0105

Released:
DOI: 10.2210/pdb5v9i/pdb
PDB entry 5v9i coloured by chain, front view.
PDB entry 5v9i coloured by chain, side view.
PDB entry 5v9i coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Dong A, Zeng H, Liu J, Xiong Y, Babault N, Jin J, Walker JR, Bountra C, Arrowsmith CH, Edwards AM, Wu H, Brown PJ, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a [histone H3]-L-lysine(9) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(9)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-lysine N-methyltransferase EHMT2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 283 amino acids
Theoretical weight: 32.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96KQ7 (Residues: 913-1193; Coverage: 23%)
Gene names: BAT8, C6orf30, EHMT2, G9A, KMT1C, NG36
Sequence domains:
Structure domains: SET domain

Ligands and Environments

5 bound ligands:
Ligand ZN 16 x ZN
Ligand SAM 4 x SAM
Ligand 90P 4 x 90P
Ligand GOL 9 x GOL
Ligand UNX 4 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 56.607Å b: 77.509Å c: 134.794Å
α: 90° β: 91.41° γ: 90°
R-values:
R R work R free
0.234 0.234 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links