› 5va7

X-ray diffraction
2.15Å resolution

Glucocorticoid Receptor DNA Binding Domain - IL11 AP-1 recognition element Complex

Released:
DOI: 10.2210/pdb5va7/pdb
PDB entry 5va7 coloured by chain, front view.
PDB entry 5va7 coloured by chain, side view.
PDB entry 5va7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tethering not required: the glucocorticoid receptor binds directly to activator protein-1 recognition motifs to repress inflammatory genes.
Weikum ER, de Vera IMS, Nwachukwu JC, Hudson WH, Nettles KW, Kojetin DJ, Ortlund EA
Nucleic Acids Res 45 8596-8608 (2017)
PMID: 28591827

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119270 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Glucocorticoid receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 70 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04150 (Residues: 419-488; Coverage: 9%)
Gene names: GRL, NR3C1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*AP*GP*GP*GP*TP*GP*AP*GP*TP*CP*AP*GP*GP*AP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 5.04 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*AP*TP*CP*CP*TP*GP*AP*CP*TP*CP*AP*CP*CP*CP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.75 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 39.143Å b: 96.855Å c: 104.554Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.227
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

A General Introduction to Glucocorticoid Biology.
Timmermans et al. (2019)
11 more

3 mentions without citation

Structural insights into glucocorticoid receptor function.
Frank et al. (2021)
2 more