› 5vai

Electron Microscopy
4.1Å resolution

Cryo-EM structure of the activated Glucagon-like peptide-1 receptor in complex with G protein

Released:
DOI: 10.2210/pdb5vai/pdb
PDB entry 5vai coloured by chain, front view.
PDB entry 5vai coloured by chain, side view.
PDB entry 5vai coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of the activated GLP-1 receptor in complex with a G protein.
Zhang Y, Sun B, Feng D, Hu H, Chu M, Qu Q, Tarrasch JT, Li S, Sun Kobilka T, Kobilka BK, Skiniotis G
Nature 546 248-253 (2017)
PMID: 28538729
Related structures: EMD-8653

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-207875 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Glucagon like peptide 1 receptor Chain: R
Molecule details ›
Chain: R
Length: 461 amino acids
Theoretical weight: 52.58 KDa
Source organism: Oryctolagus cuniculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: G1SGD4 (Residues: 24-422; Coverage: 91%)
Gene name: GLP1R
Sequence domains:
Glucagon-like peptide 1(7-37) Chain: P
Molecule details ›
Chain: P
Length: 31 amino acids
Theoretical weight: 3.36 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01275 (Residues: 98-128; Coverage: 19%)
Gene name: GCG
Sequence domains: Peptide hormone
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.37 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 96%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Rattus norvegicus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 68 amino acids
Theoretical weight: 7.56 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63212 (Residues: 1-68; Coverage: 96%)
Gene name: GNG2
Sequence domains: GGL domain
nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-8653
Expression systems:
  • Spodoptera frugiperda
  • Not provided
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

71 review citations

How Ligands Illuminate GPCR Molecular Pharmacology.
Wacker et al. (2017)
70 more

73 mentions without citation

Mechanisms of adhesion G protein-coupled receptor activation.
Vizurraga et al. (2020)
72 more