› 5vbl

X-ray diffraction
2.6Å resolution

Structure of apelin receptor in complex with agonist peptide

Released:
DOI: 10.2210/pdb5vbl/pdb
PDB entry 5vbl coloured by chain, front view.
PDB entry 5vbl coloured by chain, side view.
PDB entry 5vbl coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for Apelin Control of the Human Apelin Receptor.
Ma Y, Yue Y, Ma Y, Zhang Q, Zhou Q, Song Y, Shen Y, Li X, Ma X, Li C, Hanson MA, Han GW, Sickmier EA, Swaminath G, Zhao S, Stevens RC, Hu LA, Zhong W, Zhang M, Xu F
Structure 25 858-866.e4 (2017)
PMID: 28528775

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132351 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
agonist peptide Chain: A
Molecule details ›
Chain: A
Length: 17 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
Apelin receptor; Rubredoxin Chain: B
Molecule details ›
Chain: B
Length: 407 amino acids
Theoretical weight: 46.44 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P35414 (Residues: 7-229, 243-330; Coverage: 82%)
  • Canonical: P00268 (Residues: 1-54; Coverage: 100%)
Gene names: AGTRL1, APJ, APLNR
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand OLC 1 x OLC
4 modified residues:
Ligand HRG 1 x HRG
Ligand ALC 1 x ALC
Ligand OIC 1 x OIC
Ligand NLE 1 x NLE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 150.58Å b: 44.3Å c: 79.45Å
α: 90° β: 114.87° γ: 90°
R-values:
R R work R free
0.24 0.239 0.256
Expression systems:
  • Not provided
  • Spodoptera frugiperda

Quick links

Citations

18 review citations

Advances in therapeutic peptides targeting G protein-coupled receptors.
Davenport et al. (2020)
17 more

27 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
26 more