› 5vd4

X-ray diffraction
2.02Å resolution

CRYSTAL STRUCTURE OF HUMAN WEE1 KINASE DOMAIN IN COMPLEX WITH RAC-IV-016, a MK1775 analougue

Released:
DOI: 10.2210/pdb5vd4/pdb
PDB entry 5vd4 coloured by chain, front view.
PDB entry 5vd4 coloured by chain, side view.
PDB entry 5vd4 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Zhu J-Y, Schonbrunn E

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Wee1-like protein kinase Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 32.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30291 (Residues: 291-575; Coverage: 44%)
Gene name: WEE1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand 99J 1 x 99J
Ligand PO4 2 x PO4
Ligand EDO 3 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 50.31Å b: 43.78Å c: 64.86Å
α: 90° β: 103.04° γ: 90°
R-values:
R R work R free
0.203 0.203 0.245
Expression system: Escherichia coli BL21(DE3)

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