› 5vd5

X-ray diffraction
2.05Å resolution

CRYSTAL STRUCTURE OF HUMAN WEE1 KINASE DOMAIN IN COMPLEX WITH RAC-IV-050, a MK1775 analougue

Released:
DOI: 10.2210/pdb5vd5/pdb
PDB entry 5vd5 coloured by chain, front view.
PDB entry 5vd5 coloured by chain, side view.
PDB entry 5vd5 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Zhu J-Y, Schonbrunn E

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Wee1-like protein kinase Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 32.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30291 (Residues: 291-575; Coverage: 44%)
Gene name: WEE1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 99M 1 x 99M
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 50.24Å b: 44.58Å c: 64.65Å
α: 90° β: 102.17° γ: 90°
R-values:
R R work R free
0.195 0.195 0.234
Expression system: Escherichia coli BL21(DE3)

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