› 5vko

X-ray diffraction
1.8Å resolution

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137359 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription elongation factor SPT6 Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 25.07 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P23615 (Residues: 1247-1451; Coverage: 14%)
Gene names: CRE2, G6169, SPT6, SSN20, YGR116W
Sequence domains: SH2 domain
Structure domains: SH2 domain
DNA-directed RNA polymerase II subunit RPB1 Chain: B
Molecule details ›
Chain: B
Length: 34 amino acids
Theoretical weight: 3.68 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P04050 (Residues: 1468-1500; Coverage: 2%)
Gene names: D2150, RPB1, RPB220, RPO21, SUA8, YDL140C

Ligands and Environments

1 bound ligand:
Ligand IPA 1 x IPA
2 modified residues:
Ligand TPO 1 x TPO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2221
Unit cell:
a: 42.448Å b: 102.102Å c: 115.743Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.162 0.198
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Organization and regulation of gene transcription.
Cramer P. (2019)
11 more

3 mentions without citation

A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription.
Sdano et al. (2017)
2 more