› 5vl3

X-ray diffraction
3.1Å resolution

CD22 d1-d3 in complex with therapeutic Fab Epratuzumab

Released:
DOI: 10.2210/pdb5vl3/pdb
PDB entry 5vl3 coloured by chain, front view.
PDB entry 5vl3 coloured by chain, side view.
PDB entry 5vl3 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis of human CD22 function and therapeutic targeting.
Ereño-Orbea J, Sicard T, Cui H, Mazhab-Jafari MT, Benlekbir S, Guarné A, Rubinstein JL, Julien JP
Nat Commun 8 764 (2017)
PMID: 28970495

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-148915 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (7 distinct):
B-cell receptor CD22 Chains: Q, R, S, T
Molecule details ›
Chains: Q, R, S, T
Length: 323 amino acids
Theoretical weight: 36.8 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P20273 (Residues: 22-330; Coverage: 37%)
Gene names: CD22, SIGLEC2
Sequence domains:
Ig-like domain-containing protein Chains: A, C, E, H
Molecule details ›
Chains: A, C, E, H
Length: 222 amino acids
Theoretical weight: 24.03 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: S6B291 (Residues: 20-239; Coverage: 49%)
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chains: B, D, F, L
Molecule details ›
Chains: B, D, F, L
Length: 222 amino acids
Theoretical weight: 24.34 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8TCD0 (Residues: 19-52, 53-116, 117-239; Coverage: 99%)
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P1
Unit cell:
a: 87.11Å b: 90.22Å c: 136.632Å
α: 70.83° β: 80.81° γ: 80.99°
R-values:
R R work R free
0.281 0.28 0.295
Expression system: Homo sapiens

Quick links

Citations

29 review citations

Sialic acid-binding immunoglobulin-like lectins (Siglecs) detect self-associated molecular patterns to regulate immune responses.
Läubli et al. (2020)
28 more

3 mentions without citation

Current Status on Therapeutic Molecules Targeting Siglec Receptors.
Lenza et al. (2020)
2 more