› 5vnw

X-ray diffraction
2.6Å resolution

Crystal structure of Nb.b201 bound to human serum albumin

Released:
DOI: 10.2210/pdb5vnw/pdb
PDB entry 5vnw coloured by chain, front view.
PDB entry 5vnw coloured by chain, side view.
PDB entry 5vnw coloured by chain, top view.
Source organisms:
Primary publication:
Yeast surface display platform for rapid discovery of conformationally selective nanobodies.
McMahon C, Baier AS, Pascolutti R, Wegrecki M, Zheng S, Ong JX, Erlandson SC, Hilger D, Rasmussen SGF, Ring AM, Manglik A, Kruse AC
Nat Struct Mol Biol 25 289-296 (2018)
PMID: 29434346

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230753 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Albumin Chains: A, B
Molecule details ›
Chains: A, B
Length: 585 amino acids
Theoretical weight: 66.57 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P02768 (Residues: 25-609; Coverage: 99%)
Gene names: ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341
Sequence domains: Serum albumin family
Structure domains: Serum Albumin; Chain A, Domain 1
Nb.b201 Chains: C, D
Molecule details ›
Chains: C, D
Length: 120 amino acids
Theoretical weight: 13.35 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

4 bound ligands:
Ligand GOL 4 x GOL
Ligand PLM 2 x PLM
Ligand DAO 1 x DAO
Ligand BUA 1 x BUA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 66.338Å b: 72.15Å c: 108.513Å
α: 96.14° β: 104.6° γ: 104.02°
R-values:
R R work R free
0.222 0.221 0.256
Expression systems:
  • Saccharomyces cerevisiae
  • Escherichia coli

Quick links

Citations

46 review citations

A guide to: generation and design of nanobodies.
Muyldermans S. (2021)
45 more

5 mentions without citation

Non-covalent albumin-binding ligands for extending the circulating half-life of small biotherapeutics.
Zorzi et al. (2019)
4 more